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Deletion of RF3 increases ribosome occupancy of the mRNAs in an error-prone background. (A) Polysome profile of the error-prone rpsD strain (UD131) and its prfC deletion derivative. (B) Northern blot of the same fractions follows the distribution of two endogenous transcripts, ompA and lrpA, across the gradient. (C) Western blot of fractions from the sucrose-density gradient in A used to follow the distribution of RF1 and RF2 across the gradient. See also Figure S6.
