Figure 5 .

Nonsense suppression impairs growth of rtf1Δ rkr1Δ cells. (A) Tetrad dissections of crosses between a [psi⁻] rkr1Δ sup35-Y351C strain (KY2292) and a [psi⁻] rtf1Δ rkr1Δ SUP35 strain (KY2286). Dissections were done on YPD and incubated at 30° for 2 days. Double-mutant rtf1Δ rkr1Δ SUP35 segregants are highlighted by boxes while rtf1Δ rkr1Δ sup35-Y351C triple mutants are highlighted by circles. (B) A tetrad from A was further analyzed by fivefold serial dilution analysis on YPD and incubated at 30° for 1 day. Growth differences are less apparent on YPD on later days.