Figure 6 .

The presence of [PSI⁺] affects rkr1Δ phenotypes. (A) Wild-type (KY761), rtf1Δ (KY2211), and rkr1Δ (KY2236) strains, or these strains first passaged onto YPD + 5 mM GuHCl, as well as an rtf1Δ rkr1Δ [psi⁻] strain (KY2209), were used for dilution growth assays on YPD or YPD containing 50 µM CdCl₂, 10% EtOH, 15 mM caffeine, or 0.8 µg/ml cycloheximide and incubated at 30° for 2–6 days. “PRION+” indicates uncured cells; “prion−” indicates cells passaged on medium containing GuHCl. (B) A rkr1Δ strain (KY2236) was transformed with 2µ TRP1-marked pGPD-HSP104, pGPD-URE2, pGPD-LSM4, or empty vector (pRS424). Fivefold serial dilution analysis of these transformants was performed on SC-W or SC-W containing 50 µM CdCl₂ and incubated at 30° for 3 days. (C) rkr1Δ (KY2309) and rkr1Δ sup35-Y351C (KY2306) strains were used for 10-fold dilution analysis on YPD and YPD containing 50 µM CdCl₂ and incubated at 30° for 3 days.