Figure 5 .

A promoter in cis decreases GMR action in trans. (A) Top, schematic showing the Enhancerless construct paired with Complete^lacZ. This combination of paired alleles produces variegated GFP fluorescence when integrated at 53F or 37B. (B) Relative quantification of GMR activation of GFP in trans with and without a promoter in cis by quantitative RT-PCR. For both 53F and 37B, total levels of GFP transcript generated from the Enhancerless construct are decreased in discs with an intact promoter in cis to GMR (right column) relative to those without a promoter in cis (defined as 100%, left column). Error bars indicate 95% confidence intervals. *P < 0.05 (t-test).