Figure 6 .

Effect of nucleotides on the cosedimentation of wild-type and AAA3 E/Q dynein with microtubules. (A) Representative silver-stained gel images showing microtubule cosedimentation of dynein isolated from wild-type and AAA3 E/Q strains under varying nucleotide conditions. Mixtures were centrifuged, and the supernatant (S) and pellet (P) fractions are shown. Regions boxed in red indicate dynein sedimentation in the presence of ATP. (B) Quantification of the percentage of dynein in pellet fraction in comparison to total dynein. Cosedimentation was quantified from three independent experiments. Data are shown as mean ± SEM. Wild-type dynein cosedimentation in the presence of 1 mM ATP was significantly less than wild-type dynein cosedimentation in the other conditions examined (t-test, P < 0.05). AAA3 E/Q dynein cosedimentation in the presence of ATP was not significantly different from that under the other conditions (t-test, P > 0.05)