Figure 6 .

Endos and B55/Twins physically interact in vitro. (A) Coomassie blue-stained gel showing that loading of GST::Endos on beads was similar. (B) Autoradiogram showing that GST::Endos^S68D beads bind in vitro transcribed/translated ³⁵S-labeled B55/Twins (Tws) with higher affinity than GST::Endos, GST::Endos^S68A, or GST::Endos^S68E beads. ³⁵S-labeled microtubule star (Mts, the catalytic subunit of PP2A) and PP2A-B’ (the B56 regulatory subunit of PP2A) associate with the beads at background levels. ³⁵S-labeled Elgi, a strong Endos interactor (Von Stetina et al. 2008), was used as a positive control and GST beads as a negative control.