Table 4. The C. elegans ensa-1 gene is not essential for viability or germline proliferation.
| Genotype (n) | % embryonic lethality (n) | Average brood ± SD |
|---|---|---|
| 20° | ||
| Wild type (N2) | 0.8 (2257) | 251 ± 30 (9) |
| ensa-1(tm2810) | 0.6 (1673) | 239 ± 24 (7) |
| ensa-1(tm2810)/qDf9a | 26.5 (3556) | 274 ± 43 (12) |
| +/qDf9 | 26.1 (1233) | 206 ± 33 (6) |
| 25° | ||
| Wild type(N2) | 2.1 (2092) | 209 ± 34 (10) |
| ensa-1(tm2810) | 2.3 (1552) | 172 ± 31 (9) |
| ensa-1(tm2810)/qDf9 | 26.4 (2058) | 172 ± 39 (12) |
| +/qDf9 | 28.7 (663) | 174 ± 66 (4) |
| 25° | ||
| L4440b RNAi | 0.8 (2638) | 240 ± 39 (11) |
| ensa-1(tm2810); L4440 RNAi | 0.6 (2369) | 237 ± 41 (10) |
| ensa-1(RNAi) | 1.1 (1816) | 259 ± 32 (8) |
| ensa-1(tm2810); ensa-1(RNAi) | 0.5 (2148) | 239 ± 73 (9) |
| ensa-1(tm2810); glb-30(RNAi)c | 0.7 (1416) | 202 ± 60 (7) |
| cdc-14(RNAi) | 1.2 (1240) | 248 ± 30 (5) |
| ensa-1(tm2810); cdc-14(RNAi) | 2.0 (1424) | 237 ± 72 (5) |
| sur-6(RNAi) | 98.5 (1228) | 136 ± 41 (9) |
| ensa-1(tm2810); sur-6(RNAi) | 99.0 (848) | 106 ± 43 (8) |
a
qDf9 is a deletion encompassing ensa-1 that also removes sur-6 (endoding the B55 regulatory subunit of PP2A).
b
Empty vector control.
c
glb-30 encodes a protein of unknown function with very slight homology to ensa-1.