Fig. 2.

SLC22A5 expression is regulated by estrogen. a E2 regulation of SLC22A5 expression in a panel of breast cancer cell lines. Hormone-depleted cells were treated with vehicle/E2 for 6 h, followed by mRNA expression analysis by qRT-PCR. b Time course of E2 induction of SLC22A5 expression in MCF-7 cells. c Effects of ActD and CHX on E2 induction of SLC22A5 expression. Hormone-depleted MCF-7 cells were treated with ActD (1 μg/ml) or CHX (10 μg/ml) for 1 h before the addition of vehicle/E2. RNA was harvested 2 h after treatment with vehicle/E2. d Effect of ICI 182.780 (ICI) on E2 induction of SLC22A5 expression. Hormone-depleted MCF-7 cells were treated with ICI (1 μmol/l) for 1 h before the addition of vehicle/E2. RNA was harvested 2 h after treatment with vehicle/E2. e Effects of siRNA against ER induction of SLC22A5 expression. Hormone-depleted MCF-7 cells were transfected with siRNA against luciferase (Luc) or estrogen receptor (ER) for 36 h before the addition of vehicle/E2. RNA was harvested 2 h after treatment with vehicle/E2. Inset verification of ER knockdown by Western blot