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. Author manuscript; available in PMC: 2012 Aug 10.
Published in final edited form as: Integr Biol (Camb). 2010 Oct 5;3(1):39–47. doi: 10.1039/c0ib00053a

Fig. 6. Dox dependent osteogenic differentiation in the flow channel.

Fig. 6

C9 cells were cultured in osteogenic differentiation medium in a perfused flow channel, at a flow rate of 1 μl/min x2, for 3 weeks. Images show von Kossa staining of calcium deposition for selected conditions: (A1, A2) Perfusion medium with Dox 1ng/ml (top) / 1ng/ml (bottom); (B1, B2) Perfusion medium: Dox 0ng/ml (top) / 1ng/ml (bottom); (C1, C2) Perfusion medium: Dox 0ng/ml (top) / 0ng/ml (bottom). (A3, B3, C3) Intensity of calcium staining, obtained by pooling repeated experiments (n=3 per condition). The staining of the deposited calcium is expressed at the ratio of the normalized sum of segmented (~0.25mm) image intensity and the minimum intensity along the y axis (the width) of the chamber. Data were processed using Matlab R2007 program. Flow direction: left to right. Scale bar: 1mm.