TLG2 dependency of Eno2p secretion. (A) Secretion in strains transformed with Eno2-expressing plasmids. (B) Calculated amounts of secreted proteins, compared to the levels of Pgi1p secreted. ATG, cells transformed with pUL-ATG-EGFP; ENO2, cells transformed with pULGI2-ENO2; SG1C, cells transformed with pULSG1C; ΔTLG2, TLG2 knockout strains; wt, wild-type BY4741 strains; M, marker; Tlg2p -, cells transformed with p413-ADH (control vector); Tlg2p +, cells transformed with p413-ADH-TLG2 (plasmid for producing Tlg2p). Values are means ± standard errors of the means of ≥3 independent experiments.