Table 2.
Localization of enolase fragments conjugated to DsRed- and GFP-tagged organelle markersa
| Organelle marker | No. of cells forming foci with marker, based on fluorescence with: |
No. of cells with colocalized foci | (No. of cells with colocalized foci)/(no. of cells forming DsRed foci) (%) | |
|---|---|---|---|---|
| DsRed | GFP | |||
| Nup84p | 28 | 72 | 19 | 68 |
| Pma1p | 21 | 224 | 14 | 67 |
| Snf7p | 28 | 77 | 15 | 54 |
| Chs5p | 20 | 80 | 9 | 45 |
| Sec13p | 20 | 159 | 8 | 40 |
| Pex11p | 48 | 40 | 16 | 33 |
| Vrg4p | 41 | 198 | 15 | 27 |
| Mae1p | 72 | 27 | 1 | 3.7 |
a
These findings are illustrated in Fig. 3 (see also Fig. S3 in the supplemental material).