Fig 1.

The solubility-promoting function of Hsp90 is required for yeast growth at elevated temperatures. (A) Hsp90 constructs used in these studies. Primary structures of wild-type ΔCLDCX, and CR Hsp90 are shown. Deletions in ΔCLΔCX were limited to unstructured regions. The CR sequence is from the CL region of human Hsp90. (B) ΔCLΔCX and CR Hsp90 supported yeast viability as the sole copy of Hsp90 at 25°C. (C) ΔCLΔCX and CR Hsp90 accumulated to wild-type levels at 25°C based on Western blotting of yeast lysates. Intervening lanes were removed for presentation purposes. (D) Growth of yeast strains expressing wild-type, ΔCLΔCX, or CR Hsp90 as the sole copy of Hsp90 at 30 and 35°C.