Fig 2.
Quantification of growth defects of ΔCLΔCX Hsp90. (A) Growth of ΔCLΔCX Hsp90 in liquid culture was significantly impaired at 37°C. Experiments were performed in triplicate (on the left are results from a representative experiment) and analyzed to determine the average growth effect and the variation between replicates. (B) The steady-state level of ΔCLΔCX Hsp90 was reduced compared to that of wild-type Hsp90 at 37°C based on Western blotting of yeast lysates. Intervening lanes were removed for presentation purposes. (C) In order to increase ΔCLΔCX Hsp90 expression, we generated a high-copy expression plasmid (ΔCLΔCXHC). ΔCLΔCXHC Hsp90 was defective for growth in liquid culture at 37°C. Experiments were performed in triplicate (on the left are results from a representative experiment) and analyzed to determine the average growth effect and the variation between replicates. (D) ΔCLΔCXHC Hsp90 accumulated to wild-type levels at 37°C as determined by Western blotting of yeast lysates. Intervening lanes were removed for presentation purposes. (E) Growth rates at 37°C with 1.2 M sorbitol. For growth measurements, error bars represent standard deviations (n = 3).