Fig 3.
Solubility-promoting and ATPase activities were required in the same Hsp90 molecule for robust growth. The growth deficiency of ΔCLΔCXHC was not dominant and could be rescued by coexpression of a functional WTcoil Hsp90. In contrast, coexpression of ΔCLΔCXHC with an ATP-deficient D79Ncoil failed to support robust growth. Experiments were performed in triplicate (on the left are results from a representative experiment) and analyzed to determine the average growth effect and the variation between replicates. Experiments were performed at 37,°C and error bars represent standard deviations (n = 3). Coiled-coil constructs were used to prevent cross-dimerization.