Fig 4.
Yeast with ΔCLΔCXHC Hsp90 was deficient for galactose-induced expression and activity of v-src kinase. (A) Yeast with ΔCLΔCXHC Hsp90 had reduced v-src activity a judged by phosphotyrosine buildup and had reduced v-src levels. CR Hsp90 activated v-src to wild-type levels. Intervening lanes were removed for presentation purposes. (B) Degradation of v-src over time was monitored following the addition of the protein synthesis inhibitor CHX by Western blotting for v-src in yeast lysates. (C) Galactose-induced expression was impaired in yeast with ΔCLΔCXHC Hsp90 as monitored with the non-Hsp90 client SspB.