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Results of IFA testing of BHK-21 cells mock transfected or transfected with IVT viral RNA from various EAV cDNA clones as well as cells infected with the parental vaccine strain of EAV. At 24 h posttransfection or postinfection, cells were examined by IFA using MAb 12A4 against EAV Nsp1 protein (α nsp1) and MAb 3E2 against EAV N protein (α N) followed by Texas Red-conjugated goat anti-mouse immunoglobulin (red color). Cell nucleus was stained by Hoechst 33342 (blue color).
