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Differentiation between cloned viruses and the parental EAV MLV vaccine strain. A BspEI restriction site was introduced into the full-length cDNA clone for distinguishing the cloned virus rMLVB from the parental MLV virus. A 710-bp RT-PCR fragment containing the BspEI site was amplified and then digested with BspEI. The presence of a BspEI site resulted in fragments of 530 bp and 180 bp after digestion with BspEI.
