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Growth kinetics of cloned virus and parental virus. BHK-21 cells and EECs grown in 6-well plates were infected with the parental MLV vaccine virus and the cloned rMLVB virus at an MOI of 5. (a) At 0, 6, 12, 24, 48, and 72 hpi, supernatants were harvested and virus titers were determined by plaque assay in RK-13 cells. (b) Plaque morphology of the cloned rMLVB virus and parental MLV virus in EECs. Cells were stained with 0.2% crystal violet at 72 hpi.
