Fig 5.

Experimental vaccination and challenge of horses. Group one (n = 2) and group two (n = 6) horses were intramuscularly inoculated with PBS or vaccinated with the cloned rMLVB virus (1 × 10⁷ PFU), respectively. At 35 days postvaccination, all horses were challenged with a heterologous EAV KY84 strain (1 × 10⁷ PFU). (a) Rectal temperature after vaccination and challenge; (b) neutralizing antibody titers to EAV after vaccination and challenge; (c) virus isolation results from nasal swabs after challenge; (d) virus isolation results from PBMCs after challenge; viral loads with significant difference are indicated by the asterisk.