Table 1.
Nucleotide and amino acid differences between the parental MLV vaccine strain (ARVAC) and the full-length vaccine cDNA clones
| Plasmid or virus name | Nt (amino acid) at position: |
Restriction site BspEI | Genome replication | Infectious virus production | ||
|---|---|---|---|---|---|---|
| 3289 | 3933 | 12423 | ||||
| MLV parental virus | C (Nsp3 Ser-1022) | T (Nsp4 Ser-1237) | C (N Arg-37) | No | Yes | Yes |
| pEAVrMLV-S1022L&S1237A | T (Nsp3 Leu-1022) | G (Nsp4 Ala-1237) | C (N Arg-37) | No | No | No |
| pEAVrMLV-S1022L | T (Nsp3 Leu-1022) | T (Nsp4 Ser-1237) | C (N Arg-37) | No | No | No |
| pEAVrMLV-S1237A | C (Nsp3 Ser-1022) | G (Nsp4 Ala-1237) | C (N Arg-37) | No | Yes | Yes |
| pEAVrMLV | C (Nsp3 Ser-1022) | T (Nsp4 Ser-1237) | C (N Arg-37) | No | Yes | Yes |
| pEAVrMLVB | C (Nsp3 Ser-1022) | T (Nsp4 Ser-1237) | G (N Arg-37) | Yes | Yes | Yes |