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IFN-γ released following stimulation of whole blood with an ESAT-6/CFP-10 cocktail. IFN-γ secretion was assessed post-M. bovis challenge in BCG-vaccinated (open symbols) and nonvaccinated control (closed symbols) animals. Animals were challenged with M. bovis 12 months postvaccination, and blood was sampled and assessed biweekly. IFN-γ levels are expressed as the group mean (± standard error) plasma cytokine concentration (in pg/ml).
