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Levels of IFN-γ (A), TNF-α (B), IL-6 (C), and IL-10 (D) across study groups when autologous PBMCs are added to M. tuberculosis-infected macrophages. Macrophages from study participants were cultured for 7 days and then infected with M. tuberculosis H37Rv. Forty-eight hours later, autologous monocyte-depleted PBMCs were added to the macrophage culture. Supernatants were collected 48 h later and stored at −70°C until submission for cytokine bead array.
