Fig 5.

Fluorescence resonance energy transfer (FRET) from donor Cy3-labeled MAGI-1 PDZ domains to acceptor Cy5-labeled CAR^Ex8 C terminus. Emission spectra, binding curves (average change in maximal fluorescence intensity at 570 nm [F₀-F] of Cy3-PDZ domains and Cy5 CAR^Ex8 C terminus upon excitation of Cy3 at 550 nm with increasing concentrations of Cy5 CAR^Ex8 C terminus [0 to 500 nM]), and double-reciprocal linear plot of binding curves are shown. (A) Cy3-PDZ1 and Cy5 CAR^Ex8. (B) Cy3-PDZ3 and Cy5-CAR^Ex8. (C) Cy3-PDZ2 and Cy5-CAR^Ex8 (no interaction). Data represent averages of the results of 3 separate experiments.