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. 2012 Sep;86(17):9285–9296. doi: 10.1128/JVI.00712-12

Fig 8.

Fig 8

Lytic cycle is induced in cocultured BL cells. (A) Vero-E6 cells were cocultured with Akata− EBV-eGFP cells in the absence of αhIgG for 24 h (top, right). As a control, Akata− EBV-eGFP cells were treated in the presence (top middle) or absence of αhIgG (top left). Akata− EBV-eGFP cells were harvested, and the induction of the lytic cycle was analyzed by FISH using Cy3-labeled probe for the EBV genome (top). Enlarged images of the cell in latent (bottom left) or lytic (bottom right) phase are shown. The nucleus was counterstained with DAPI. Scale bars, 100 μm. (B) The percentages of Akata− EBV-eGFP cells undergoing the lytic cycle were analyzed by a confocal laser scanning microscope. The experiment was performed three times independently. The averages and standard deviations are shown for each condition. **, P < 0.01 versus the respective control (Student's t test). (C) The expression of gp350 in cocultured BL cells. The expression of gp350 was analyzed by flow cytometry. The experiment was performed three times independently. The averages and standard deviations are shown for each condition. **, P < 0.01 versus the respective control (Student's t test).