Skip to main content
. 2012 Sep;86(17):9297–9310. doi: 10.1128/JVI.00506-12

Fig 7.

Fig 7

Cellular immune response in woodchucks immunized with pCGWHc plasmid or adenoviral vectors. (A) Dot plots of PBMCs from woodchucks 58063 and 70096 immunized three times with DNA vaccine, pCGWHc. Dot plots of PBMCs from woodchucks 46949 and 46957 immunized with recombinant AdVs after priming with Ad5WHc (B) and boosting with Ad35WHc (C). CTL response was evaluated by a CD107a degranulation assay in woodchuck PBMCs expanded in vitro for 3 days with WHcAg-derived epitope c96-110 2 weeks after the immunization. Cells stimulated with unrelated CMV-derived peptide served as controls. The cells were gated on the lymphocyte population, 7AAD, and CD4 cells. Values show the percentages of CD107a+ CD3+ CD4 T cells within the CD3+ CD4 T-cell population. (D) Summary of degranulation responses after immunization with pCGWHc plasmid or with adenoviral vectors at the time point of WHV challenge. (E) The lymphoproliferative responses of PBMCs from immunized woodchucks at the time point of WHV challenge. The PBMCs were stimulated for 5 days with a panel of 10 WHcAg-specific peptides in triplicates. The cells were then pulsed with [2-3H]adenine for 16 h, and the incorporation of [2-3H]adenine was measured. Stimulation index (SI) of ≥3.0 was considered significant. The peptides which gave the positive proliferation responses are shown above the bars.