Fig 3.

Cyclin B1 overexpression does not interfere with IE1/IE2 gene expression. U373 cells were stably transduced with lentiviral expression vectors for HA-tagged wt forms of cyclin A2 and cyclin B1, as indicated. (A) After puromycin selection, the expression of cotransduced EGFP was controlled by flow cytometry and compared to that of nontransduced cells. (B) Protein levels of exogenous HA-tagged cyclins and the corresponding endogenous cyclins were compared by immunoblot analysis. (C) Five hours after HCMV infection, cells were analyzed for IE1/IE2 protein expression and DNA content as described in the legends to Fig. 1D and G.