Fig 5.

Cyclin A2-CDK activity has a long-lasting negative effect on the early and late phases of HCMV infection. U373 cells were lentivirus transduced as indicated and enriched in G₁ phase by density arrest before HCMV infection. (A) The cell cycle distributions at the time of infection and at the indicated time points postinfection were analyzed by flow cytometry. Shown are DNA histograms of propidium iodide-stained cells (n is the haploid number of chromosomes). (B) After infection, cells were harvested at regular intervals and analyzed for expression of immediate-early (IE1/2), early (gB), and late (pp28) proteins by flow cytometry. (C) In parallel, virus growth curves were obtained by determining the number of infectious particles in the cell culture supernatants. (B and C) Means and standard deviations of three independent experiments (until 96 h p.i.), all of them using an MOI of approximately 10. From 120 to 240 h p.i., the values represent averages from two of these experiments. (D) The experiment was repeated using an MOI of approximately 0.1. Shown are growth kinetics, where the data points show the mean values of biological triplicates and the error bars indicate standard deviations.