Fig 6.

Cyclin A2 has a stronger effect on IE2 than on IE1 expression. The indicated U373 derivatives were grown to confluence and infected with HCMV using an MOI of approximately 10. (A) The expression of selected viral gene products and of exogenous, HA-tagged cyclin A2 was monitored by immunoblot analysis from 5 to 96 h p.i. (B) IE1 and IE2 protein expression was analyzed in more detail by using exon 4 (IE1 [top])-, exon 5 (IE2 [second panel from top])-, and exon 3 (IE1 and IE2 [third panel from top])-specific antibodies. The relative molecular masses of the different splice variants are indicated. As the 55-kDa form of IE2 lacks the exon 3-encoded N terminus of IE1 and IE2 full-length proteins, it appears only in the exon 5-specific blot. (A and B) Equal protein content of samples was controlled by Coomassie staining (bottom gels). Shown are representative results from three independent experiments.