Fig 7.

Sustained inhibition of IE2 mRNA accumulation by cyclin A2-CDK. U373 parental cells and the indicated lentivirus-transduced derivatives were density arrested and HCMV infected. At the indicated times postinfection, total RNA was prepared from infected cells and analyzed for IE1, IE2, and UL112/113 mRNA expression by quantitative real-time PCR. The results are shown as fold change of mRNA expression compared to the values of nontransduced cells at 5 h p.i. Presented are the means and standard deviations of technical triplicates. Where no standard deviation is indicated, the means of technical duplicates are shown.