Fig 3.

In cell association of HMGB1 and HMGB2 with influenza virus NP detected in a protein complementation assay. 293T cells were cotransfected with the indicated combinations of the Luc1-fusion or control Luc1 expression plasmid on one hand and the Luc2-fusion or control Luc2 plasmid on the other hand. The viral fusion proteins were derived from the A/WSN/33 strain, or from the pandemic (pdm) A/Paris/2590/2009 virus when indicated. Cell extracts were prepared at 24 h posttransfection and tested for Gaussia princeps luciferase activity. The normalized luminescence ratios (NLR) as defined by Cassonnet et al. (15) were expressed as percentages and as means ± the SD of triplicates. The dotted line represents the maximum NLR measured with the negative control plasmids.