Fig 4.

Localization of influenza virus NP in infected MDCK cells treated with (panels A to D) SA-19 or reference compounds or (panels F to I) analogues of SA-19. MDCK cells were infected with A/X-31 influenza virus (MOI: 4 PFU per cell) in the presence of compound and incubated for 1 h at 35°C. After fixation and permeabilization, the cells were stained with anti-NP antibody and Alexa Fluor 488-labeled secondary antibody (green). The nuclei were visualized with DAPI (blue). Bar, 25 μm. See Fig. 1 for the chemical structures of (aglyco)ristocetin, SA-19, SA-48, and SA-26.