Fig 5.

CD4⁺ T cell subsets in spleen, mesenteric, and inguinal LN. (A) Gating strategies for CD4⁺ T cell subsets. (Top) CCR5⁺ CD4⁺ T cells were gated into four CXCR3/CCR6 subsets: CXCR3+ CCR6⁻, CXCR3⁻ CCR6⁻, CXCR3⁺ CCR6⁺, and CXCR3⁻ CCR6⁺ cell. (Middle) Gating for central memory (CM), transitional memory (TrM), and effector memory (EM) subsets. (Bottom left) Gating for CCR7⁻ CCR5⁺ and CCR7⁻ CCR5⁺ subsets. (Bottom right) Gating for CXCR3⁻ CCR5⁺ and CXCR3⁺ CCR5⁺ subsets. The TrM, CXCR3⁺ CCR5⁺, and CCR7⁻ CCR5⁺ subsets are indicated by arrows. (B, C, and D) Frequencies of the TrM, CXCR3⁺ CCR5⁺, and CCR7⁻ CCR5⁺ subsets in CD4⁺ memory T cells from the spleen (B), mesenteric LN (C), and inguinal LN (D) were compared between Δ5G-infected animals at 9 to 14 days p.i. and SIVmac239-infected animals at 7 to 14 days p.i. The significance of differences was determined by using the unpaired Student t test. The frequencies in the uninfected animals are shown as references. The differences implicate background immune responses in the corresponding animal. (E) Percentages of four CXCR3/CCR6 subsets in CCR5⁺ CD4⁺ T cells from the mesenteric LN (left) and inguinal LN (right) of Δ5G- and SIVmac239-infected animals. The percentages of the CXCR3⁺ CCR6⁻ cells from these tissues of the SIVmac239-infected animals at 9 to 12 days p.i. were significantly lower than those in the uninfected animals, Δ5G-infected animals, and SIVmac239-infected animals at 7 days p.i.