Fig 6.

CD4⁺ T cell subsets in the small intestinal tissues. (A) Flow profiles of CD4⁺ T cell subsets in the ileal tissues from an uninfected animal (upper panel), a Δ5G-infected animal at 9 days p.i. (lower left), and a Δ5G-infected animal at 12 days p.i. (lower right). The CD4⁺ CD8⁻ T cells and CD4⁺ CD8⁺ T cells were gated as indicated. (Top) CM, TrM, and EM subsets. (Middle) CCR5⁺ TrM and CCR5⁺ EM subsets. (Bottom) CXCR3/CCR6 subsets in CCR5⁺ cells. (B) Frequencies of CXCR3/CCR6 subsets in CCR5⁺ CD4⁺ T cells from jejunal tissues (left) and ileal tissues (right) from uninfected animals, Δ5G-infected animals, and SIVmac239-infected animals. The flow analysis was based on the gating strategy shown in Fig. 5A (top profiles). Although the CXCR3⁺ CCR6⁻ subset (blue) was predominant in uninfected animals, a remarkably higher CXCR3⁻ CCR6⁺ subset (yellow) from these tissues of Δ5G-infected animals at 9 days p.i. was noted.