Fig 5.

The NF-κB inhibitor IKK2 VI counteracts TNF-α-induced reactivation of latent virus in HPCs. (A, C, and E) Flow cytometric analysis of CD133⁺ cells infected using the indicated envelope, sorted to remove actively infected cells, and stimulated overnight as indicated. Live cells were gated using forward scatter (FSC), side scatter (SSC), and 7-AAD; numbers indicate the percentage of cells within each gate. Results are representative of 4 (A) or 2 (C and E) independent experiments. (B, D, and F) Summary of reactivation for panels A, C, and E, respectively. The fold increase in the percentage of cells expressing PLAP over that for cells incubated in STIF medium plus DMSO was calculated. Means and standard errors for 4 (B) or 2 (D or F) independent experiments are shown. Asterisk indicates a significant difference (P, <0.01 [B], <0.02 [D], or <0.03 [F]) by a paired t test. (G) Quantitation of nuclear NF-κB p50 DNA binding activity for cells treated as for panel A and analyzed as described for Fig 4A. Means and standard errors for two experiments are shown.