Fig 6.

NGF-dependent cell cycle reentry and apoptosis is independent of cdk4/6 activity. (A) Dose-response curve of the effect of the selective cdk4/6 inhibitor PD 0332991 on the capacity of DCRNs to incorporate BrdU after a short pulse (30 min). The analysis was performed after 16 h of inhibitor treatment. Each experimental point is shown as mean ± the SEM (n = 3). (B) Luciferase activity, normalized to β-galactosidase, in extracts from DCRNs transfected with c-Myc-luciferase and β-galactosidase and treated for 16 h with different combinations of NGF and the cdk4/6 selective inhibitor PD 0332991. (C and D) BrdU incorporation (30-min pulse) (C) and proportion of apoptotic nuclei (D) in DCRNs treated for 16 h with different combinations of NGF and the cdk4/6 selective inhibitor PD 0332991. *, P < 0.05; ***, P < 0.005 (Student t test; n = 3).