Fig 3.

Subcellular distribution of phospho-MeCP2. (A) Immunofluorescent staining of the nucleus of a cortical neuron from 5-week-old mice with the indicated antibodies. DAPI was used to visualize heterochromatic foci (chromocenters). Bar, 5 μm. (B) Magnification of the region indicated by boxes in panel A containing a single heterochromatic chromocenter. Bar, 1 μm. (C) Gel electrophoresis of DNA from MNase fractions from SH-SY5Y nuclei. (D) Western blot analysis of MNase fractions from SH-SY5Y nuclei with antibodies against RNA polymerase II, MeCP2, and histone H1. (E) Western blot analysis of MNase nuclear fractions from SH-SY5Y cells stably expressing MeCP2e1-FLAG with the indicated antibodies.