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. 2012 Jul;32(14):2894–2903. doi: 10.1128/MCB.06728-11

Fig 4.

Fig 4

pS80 and pS229 MeCP2 interact specifically with unique MeCP2 binding partners. (A) Western blot of proteins immunoprecipitated from SH-SY5Y cell nuclear extracts with antibodies against total, pS80, or pS229 MeCP2 or nonspecific IgY. Identical blots were performed using equal volumes of precipitate (left) or equal loading of MeCP2 (right). The percentage of each precipitate loaded is indicated below each lane. (B) Western blot of proteins immunoprecipitated from SH-SY5Y nuclear extracts which had been mock treated or treated with Benzonase (B) or RNase A and T1 (R) prior to immunoprecipitation with antibodies against pS80 or pS229 MeCP2 or nonspecific IgY. The percentage of each precipitate used is indicated below each lane. (C) Coomassie staining of SDS-PAGE-separated proteins from a pulldown assay using GST-tagged wild-type and mutant MeCP2e1. (D) Western blot of proteins from panel C with the indicated antibodies. (E) Western blot of proteins from a pulldown assay from SH-SY5Y nuclear extract using GST-tagged wild-type and mutant MeCP2e1 with the indicated antibodies.