Fig 5.

MeCP2 regulates RA-induced expression of RET. (A) Changes in gene expression induced by treatment of SH-SY5Y cells with 10 μM RA for the indicated times. (B) Schematic of the RET gene showing the qPCR amplicons used for panels C to E. (C to E) ChIP with total MeCP2 (C), pS80 MeCP2 (D), and pS228 MeCP2 (E) antibodies analyzed by quantitative PCR at the indicated sites, expressed as enrichment over nonspecific IgY. (F) RA-induced changes in expression of RET in parental SH-SY5Y cells or SH-SY5Y cells stably expressing the indicated MeCP2e1-FLAG construct. Expression was measured by quantitative PCR and normalized to GAPDH expression. Data are means from three independent biological replicates. Error bars represent standard errors of the means (SEM). *, P < 0.05 compared to the corresponding result in the SH-SY5Y parental cell line.