Fig 5.

Cot contributes to osteoclastogenesis through Ca²⁺ oscillation/calcineurin-independent osteoclast differentiation in vivo. (A) The number of TRAP-positive MNCs lacking both IP₃R2 and Cot kinase (IP₃R2/CotKO) was significantly decreased compared to those in WT and IP₃R2KO mice in a coculture system. n = 3 for each group. Values are means ± SD. **, P < 0.01. Bar, 100 μm. (B) Histology (TRAP and toluidine blue staining) of the tibias of IP₃R2KO and IP₃R2/CotKO mice at 10 weeks of age. Bars, 100 μm. (C) Histomorphometric parameters of osteoclastic bone resorption and bone volume in the tibias of IP₃R2KO and IP₃R2/CotKO mice at 10 weeks of age. Values are means ± SD. **, P < 0.01. N.S., difference was not significant. n = 4 for each group.