Fig 4.

SPT inhibition induces activation of Hog1 via both the Sln1 and the Sho1 branches. ssk2Δ ssk22Δ is abbreviated as ssk2,22Δ. (A) Wild-type (TM141, WT), lcb2-M1 (MH280), lcb2-M1 ssk2,22Δ (MH317), lcb2-M1 sho1Δ (MH319), lcb2-M1 ste11Δ (MH322), and lcb2-M1 ssk2,22Δ sho1Δ (MH321) cells were subjected to Western blotting for phospho-Hog1 and total Hog1. (B) Wild-type (TM141, WT), ssk2,22Δ (TM252), ste11Δ (MH273), and ssk2,22Δ ste11Δ (MH276) cells were treated with 2 μg/ml myriocin for 3 h and then subjected to Western blotting as in panel A. lcb2-M1 (MH280) cells were used as a positive control.