Fig 7.

Filipin treatment affects activation of the HOG pathway. (A) Filipin treatment impedes activation of the Sho1 branch. Wild-type (TM141, WT), ssk2,22Δ (TM252), and sho1Δ (TM287) cells were treated or not treated with 5 μg/ml filipin for 5 min and then treated with 0.4 M KCl. After 5 min of incubation, cells were collected and subjected to Western blotting. (B) Filipin treatment after exposure to osmotic stress sustains activation of the HOG pathway. ssk2,22Δ (TM252) and sho1Δ (TM287) cells were exposed to 0.4 M KCl and incubated for 5 min (0 min). Then, cells were treated with 5 μg/ml filipin, incubated for the indicated times, and collected and subjected to Western blotting. (C) Filipin treatment induces activation of the Sln1 branch. ssk2,22Δ (TM252) and sho1Δ (TM287) cells were treated with 5 μg/ml filipin for the indicated times and subjected to Western blotting.