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. 2012 Jul;32(14):2861–2870. doi: 10.1128/MCB.06111-11

Fig 8.

Fig 8

The lcb2-M1 mutation causes activation of Kss1 and Fus3 in a Sho1-independent but Ste11-dependent manner. Wild-type (TM141, WT), lcb2-M1 (MH280), lcb2-M1 ssk2,22Δ (MH317), lcb2-M1 sho1Δ (MH319), lcb2-M1 ste11Δ (MH322), and lcb2-M1 ssk2,22Δ sho1Δ (MH321) cells were subjected to Western blotting using anti-phospho-p38 (which detects phospho-Hog1), anti-Hog1, anti-phospho-p44/p42 MAPK (which detects phospho-Kss1 and phospho-Fus3), and anti-Kss1 antibodies.