Table 1.
Yeast strains used in this study
| Strain | Genotype | Reference or source |
|---|---|---|
| TM415 | MATa leu2Δ1 trp1Δ63 his3Δ200 ade2 URA3::(lexAop)8-lacZ LYS2::(lexAop)4-HIS3 | L40-derived lab stock |
| TM415α | Same as TM415 except for MATα | Lab stock |
| MH266 | Same as TM415α except for lcb2-M1 | This study |
| TM100 | MATa ura3-52 leu2Δ1 trp1Δ63 | 17 |
| TM101 | MATα ura3-52 leu2Δ1 his3Δ200 | 18 |
| TM141 | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 | 17 |
| TM221 | MATα ade8 | Lab stock |
| TM225 | MATα ura3-52 leu2Δ1 his3Δ200 lys2Δ202 | 11 |
| TM252 | MATa ura3-52 leu2Δ1 trp1Δ63 ssk2::LEU2 ssk22::LEU2 | 18 |
| TM287 | MATα ura3-52 leu2Δ1 trp1Δ63 his3Δ200 sho1::HIS3 | Lab stock |
| TM370 | MATα ura3-52 leu2Δ1 trp1Δ63 his3Δ200 ssk2::LEU2 ssk22::LEU2 sho1::TRP1 | Lab stock |
| TM537 | MATa ura3-52 leu2Δ1 trp1Δ63 erg3::His3MX6 | Lab stock |
| TM540 | MATa ura3-52 leu2Δ1 trp1Δ63 erg6::His3MX6 | Lab stock |
| 4007 | MATa ura3Δ0 leu2Δ0 his3Δ1 met15Δ0 ipt1::kanMX4 | Open Biosystems |
| 5281 | MATa ura3Δ0 leu2Δ0 his3Δ1 met15Δ0 sur4::kanMX4 | Open Biosystems |
| MH273 | MATα ura3-52 leu2Δ1 his3Δ200 ste11::URA3 | This study |
| MH276 | MATa ura3-52 leu2Δ1 trp1Δ63 ssk2::LEU2 ssk22::LEU2 ste11::loxP-kanMX-loxP | This study |
| MH280 | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lcb2-M1 | This study |
| MH293 | MATa ura3-52 leu2Δ1 trp1Δ63 elo1::kanMX4 | This study |
| MH296 | MATa ura3-52 leu2Δ1 trp1Δ63 csg1::kanMX4 | This study |
| MH302 | MATα ura3-52 leu2Δ1 his3Δ200 csh1::kanMX4 | This study |
| MH305 | MATa ura3-52 leu2Δ1 trp1Δ63 sur2::kanMX4 | This study |
| MH309 | MATα ura3-52 leu2Δ1 trp1Δ63 csg1::kanMX4 csh1::kanMX4 | This study |
| MH311 | MATa leu2Δ0 his3Δ1 met15Δ0 sur4::kanMX4 | This study |
| MH314 | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 fen1::kanMX4 | This study |
| MH317 | Same as MH280 except for ssk2::LEU2 ssk22::LEU2 | This study |
| MH319 | Same as MH280 except for sho1::TRP1 | This study |
| MH321 | Same as TM370 except for lcb2-M1 | This study |
| MH322 | Same as MH280 except for ste11::URA3 | This study |
| MH324 | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 SLN1-EGFP::His3MX6 | This study |
| MH329 | MATα ura3-52 leu2Δ1 trp1Δ63 his3Δ200 SHO1-GFP::His3MX6 lcb2-M1 | This study |
| MH331 | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 SLN1-9myc::His3MX6 | This study |
| MH335 | MATα ura3-52 leu2Δ1 trp1Δ63 his3Δ200 SLN1-9myc::His3MX6 SHO1-GFP::His3MX6 | This study |
| MH337 | Same as MH335 except for lcb2-M1 | This study |
| MH417 | MATα ura3-52 leu2Δ1 his3Δ200 lys2Δ202 erg3::His3MX6 | This study |
| MH421 | MATa ura3-52 leu2Δ1 his3Δ200 lys2Δ202 lcb2-M1 erg3::His3MX6 | This study |
| MH425 | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 erg2::His3MX6 | This study |