Fig 3.

LAT in CTL cytotoxicity in vitro. (A) In vitro cytotoxicity against EL4 target cells. The data shown are from one representative of five independent experiments. (B) IFN-γ production and FasL upregulation. CTLs were restimulated by EL4 cells preloaded with (open) or without (filled) Ova peptide for 5 h. Intracellular staining of IFN-γ and surface expression of FasL on CD8⁺ cells were analyzed by FACS. The data are representative of three independent experiments. (C) In vitro cytotoxicity against L1210/K^b target cells. The data shown are from one representative of three independent experiments. (D) Granzyme B (GzmB) and perforin expression by Western blotting. The data are representative of three independent experiments. (E) Delivery of granules. L1210/K^b cells were labeled with Cell Tracker Orange with or without Ova peptide. CTLs were incubated with L1210/K^b cells in the presence of a cell-permeable fluorescein isothiocyanate-conjugated gzmB substrate for 40 min. The numbers in the figure indicate the percentage of L1210/K^b cells that received gzmB from CTLs. The data are representative of two independent experiments. n.s., not significant; *, P < 0.05; **, P < 0.005. Error bars represent means ± the standard errors of the mean (SEM).