Fig 1.

Skeletal muscle predominantly expresses the AMPK β2 subunit. (A) Immunoblot analysis using β1/β2 C-terminus-specific antibody detects expression of β1 and β2 in WT tissues. (B) Immunoblot analysis of type I (soleus and quadriceps), type II (tibialis), and mixed (gastrocnemius) skeletal muscle and cardiac muscle using β1/β2 C-terminus-specific antibody. (C) Numbers 1 to 7 are exons of the β2 gene; a, b, and c are genotyping primers for wild-type and mutant β2 alleles. SA, splice acceptor; β-Geo, β-galactosidase-neomycin resistance cassette; PA, polyadenylation signal. (D) Genotyping PCR of tail DNA from β2 mutant (−/−), WT (+/+), and heterozygote (+/−) animals. (E) Immunoblot analysis using β1/β2 C-terminus-specific antibody showing the absence of β2 subunit expression in all organs of β2 mutant mice.