Fig 6.

Hho1 and Ume6 are depleted during meiosis and show a high genome-wide binding correlation. (A) Hho1 and Ume6 proteins are depleted with the same kinetics at the onset of meiosis (2 to 4 h after induction of sporulation). Western blot analysis of protein extracts from yeast harvested at different times over sporulation was carried out for Hho1 and Ume6. (B) Quantification of the Western blot in panel A. Hho1 and Ume6 levels are normalized to H4. (C to F) A ChIP-chip study of many chromatin-associated proteins in vegetatively growing yeast (50) was analyzed for binding correlations between various factors. Hho1 and Ume6 binding is well correlated at the promoters of all genes (C) but is most highly correlated at the promoters of early meiotic genes (D). Such a high correlation is not seen for Ume6 and Rpd3, a known corepressor in the Ume6 complex (E), or for Hho1 and Rfx1, a strong transcriptional repressor of DNA damage-regulated genes (F).