Fig 7.

Hho1 enrichment at meiotic gene promoters is Ume6 dependent. (A) Western blot analysis of protein extracts from WT, hho1Δ, and ume6Δ strains shows that Hho1 protein levels are comparable in WT and ume6Δ yeasts growing in glucose or acetate medium. Total histone H4 is used as a loading control. (B) ChIP-qPCR of Hho1 shows a decrease in Hho1 binding in ume6Δ cells specifically at promoters (Prom) of early meiotic genes repressed by Ume6 (HOP1, MEI4, PCH2, and REC104). GAPDH is a constitutively active housekeeping gene, and SMK1 is a middle sporulation gene that is not repressed by the Ume6 complex. (C) ChIP-qPCR of Hho1 shows that it is enriched at the promoters of early meiotic genes before, but not during, meiosis. Cells were cross-linked in presporulation medium (0 h), during meiosis (4 h), and in mature spores (48 h).