Fig 2.
Overproduction of YknW, YknX, and YknYZ in B. subtilis. (A) Membrane fractions isolated from HB6127 (yknWXYZ∷Kan) carrying the indicated plasmids were separated by 12% SDS-PAGE and stained with CBB (top panel) or electroblotted onto a polyvinylidene difluoride (PVDF) membrane and probed with anti-YknX antibodies (bottom panel). The expression of yknWXYZ was induced by addition of 0.5% xylose for 1 h at 37°C. (B) RT-PCR analysis of total mRNA isolated from HB6186 [Pspac-hy-sdpABC (Cm) sdpI∷mls yknWXYZ∷Kan] carrying pHTXYZ or pHTWXYZ with primers complementary to yknY and yknZ transcripts.