Table 1.
Strains and plasmids
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| BL21(DE3) | F− ompT hsdSB(rB− mB−) gal dcm | Novagen |
| DH5α | supE44 ΔlacU169 hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | |
| B. subtilis | ||
| 168 | trpC2 | P. Klebba |
| Cu1065 | trpC2 attSPβ | 3 |
| HB6098 | Cu1065 Pspac-hy-sdpABC (Cm) | 3 |
| HB6121 | Cu1065 sdpABC-sdpRI∷tet yknW∷Kan | 3 |
| HB6127 | Cu1065 yknWXYZ∷Kan | 3 |
| HB6129 | Cu1065 sdpABC-sdpRI∷tet yknWXYZ∷Kan | 3 |
| HB6178 | Cu1065 sdpABC-sdpRI∷tet yknYZ∷Kan | 3 |
| HB6182 | Cu1065 sdpI∷mls yknWXYZ∷Kan | 3 |
| HB6186 | Cu1065 Pspac-hy-sdpABC (Cm) sdpI∷mls yknWXYZ∷Kan | 3 |
| HB6349 | Cu1065 yknW∷Kan | 3 |
| Plasmids | ||
| pET21d(+) | E. coli cloning vector | Novagen |
| pDG1514 | Contains tetracycline resistance gene (tet) | BGSC ECE100 |
| pEPR | pET21d(+) carrying processed SdpC | This study |
| pETXhis | pET21d(+) carrying yknXhis | This study |
| pETWhis | pET21d(+) carrying yknWhis | This study |
| pHCMC04 | E. coli and B. subtilis shuttle vector | BGSC ECE189P |
| pHTCMC04 | pHCMC04 carrying tet from pDG1514 | This study |
| pHX | pHCMC04 carrying yknX | This study |
| pHXHis | pHCMC04 carrying the 6His-tagged yknX | This study |
| pHWX | pHCMC04 carrying yknWX | This study |
| pHWXHis | pHCMC04 carrying yknW and the 6His-tagged yknX | This study |
| pHW | pHCMC04 carrying yknW | This study |
| pHWHis | pHCMC04 carrying the 6His-tagged yknW | This study |
| pHTWX | pHTCMC04 carrying yknXYZ | This study |
| pHXYZ | pHCMC04 carrying yknXYZ | This study |
| pHXYZHis | pHCMC04 carrying yknXY and the 6His-tagged yknZ | This study |
| pHTXYZ | pHTCMC04 carrying yknXYZ | This study |
| pHYZ | pHCMC04 carrying yknYZ | This study |
| pHYZHis | pHCMC04 carrying yknY and the 6His-tagged yknZ | This study |
| pHWXYZ | pHCMC04 carrying yknWXYZ | This study |
| pHWXYZHis | pHCMC04 carrying yknWXY and 6His-tagged yknZ | This study |
| pHTWXYZ | pHTCMC04 carrying yknWXYZ | This study |