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Inactive DGC does not suppress ΔfimX pilus assembly defects. ΔfimX expressing PA1120 or the enzymatically inactive allele PA1120m was harvested from plates supplemented with 0.2% arabinose. Total surface pilin was measured by ELISA. Bars indicate means ± SD of triplicate samples from three independent experiments. One-way ANOVA followed by Dunnett's comparison test was used to compare values to the vector control, ΔfimX/pMQ72 (VC). *, P < 0.05; **, P < 0.01; ***, P < 0.001.
